A097
A003Stearic Acid
| Column | Kromasil ODS, 5 µ (250 x 4.6 mm) |
|---|---|
| Eluent | A : 0.1% Aqueous TFA/MeOH (90/10) B : Methanol/Acetone (60/40) |
| Flow Rate | 1.0 ml/min |
| Injected Volume | 200 µg |
| Peaks | 1. Linolenic acid (18:3) 2. Linoleic acid (18:2) 3. Oleic acid (18:1) + palmitic acid (16:0) 4. Stearic acid (18:0) 5. δ-Tocopherol 6. γ,β-Tocopherols 7. α-Tocopherol 8. Stigmasterol |
| Column | Zorbax ODS 5-7 µm (alkyl bonded silica) 150 x 4.6 mm |
|---|---|
| Eluent | CO2/Methanol (97.6/2.4) |
| Flow Rate | 3.9 ml/min |
| Injected Volume | 700 ng of each compound in 4 µl THF |
| Peaks | 1. Lauric acid (C12) 2. Myristic acid (C14) 3. Palmitic acid (C16) 4. Stearic acid (C18) 6. Docosanoic acid (C22) 7. Tetracosanoic acid (C24) |