AN1-5
AN1-2Rebaudioside A (from Stevia)
Application Number : AN1 (2 methods)
| Column | Ascentis Express HILIC 2.7 µm 2.1 x 150 mm, 30 °C |
|---|---|
| Eluent | A. Ammonium formate 200 mM pH 3 / B. Acetonitrile |
| Gradient | 0 to 1 min: 8% A, 1 to 8 min: 8 to 25% A, hold 4 min |
| Flow Rate | 0.5 ml/min |
| Injected Volume | 1 µl (500 to 1000 ppm each) |
| Detector | SEDEX LT-ELSD 90LT, 40 °C, 3.5 bar |
| Column | Hypersil GOLD HILIC 1.9 µm 2.0 x 150 mm, 40 °C |
|---|---|
| Eluent | A. Ammonium acetate 50 mM pH 5 / B. Acetonitrile |
| Gradient | 0 to 0.5 min: 5% A, 0.5 to 10 min: 5 to 30% A |
| Flow Rate | 0.5 ml/min |
| Injected Volume | 2 µl (500 ppm each) |
| Detector | SEDEX LT-ELSD 90LT, 35 °C, 3.5 bar |
| Peaks | Erythritol (2.45 min) Sucralose (3.61 min) Sorbitol (5.47 min) Neotame (6.04 min) Acesulfame K (6.79 min) Neohesperidin dihydrochalcone (7.35 min) Saccharin (7.92 min) Aspartame (8.45 min) Cyclamate (8.80 min) Rebaudioside A (9.68 min) |