A113
A114
A134Proline
| Column | RP8 Spherisorb (100X4.6 mm ID) |
|---|---|
| Eluent | Step elution gradient: H2O (+TFA) to 20% ACN (+TFA) |
| Flow Rate | Flow rate gradient |
| Injected Volume | - |
| Column | Silica-C8 ( 125X4.6 mm ID) |
|---|---|
| Flow Rate | 1 ml/min |
| Injected Volume | - |
| Column | Chirobiotic T 250 x 4.6 mm |
|---|---|
| Eluent | Acetonitrile / Water (80/20) |
| Flow Rate | 1 ml/min |
| Injected Volume | 5 µl (1.5 mg/l) |