A054
A088Polyethylene Glycol PEG 1450
| Column | Nucleosil 120-7-C18 250 x 4 mm. |
|---|---|
| Eluent | A: Water/Methanol (95/5) [w/w] B: Methanol/Water (95/5) [w/w] |
| Flow Rate | 1.0 ml/min |
| Injected Volume | - |
| HPLC (top chromatogram) | |
|---|---|
| Column | Nucleosil C18 120-7 250 x 4 mm |
| Eluent | A: Water/Methanol (95/5) B: Methanol/Water (95/5) (v/v) |
| Gradient | 20% to 60% B in 30 min, then hold |
| Flow Rate | 1.0 ml/min |
| Temperature | 36 °C |
| SFC (bottom chromatogram) | |
| Column | Pecosphere Silica 30 x 36 mm |
| Eluent | CO2/Methanol/Et3N (79.8/18/9/0.1) |
| Flow Rate | 5 ml/min |
| Injected Volume | 20 µl |
| Pressure | 235 bars |
| Temperature | 45 °C |