A001
A008
A011
A044
A074
A075
A077
A143
A010
A049
A050
AN1-1Fructose
Application Number : AN1 – AN2
| Column | Polypore Ca+2 and Zorbax ODS - 7 µm 250 x 4.6 mm |
|---|---|
| Eluent | Water |
| Flow Rate | 1.0 ml/min |
| Injected Volume | 20 µl |
| Column | Nucleosil NH2 - 5 µm 250 x 4.6 mm |
|---|---|
| Eluent | CH3CN/H20 (80/20) |
| Flow Rate | 2 ml/min |
| Injected Volume | 10 µl |
| Peaks | F. Fructose G. Glucose S. Sucrose |
| Column | Lichrospher Diol - 10 µm 250 x 7 mm |
|---|---|
| Eluent | A: Acetonitrile, B: Water |
| Flow Rate | 1.0 ml/min |
| Injected Volume | 20 µl |
| Peaks | 1. Fructose 2. Glucose 3. Sucrose (saccharose) 4. Lactose 5. Raffinose 6. Dextrans |
| Column | HPX 87H |
|---|---|
| Eluent | Water |
| Flow Rate | 0.5 ml/min |
| Injected Volume | 20 µl, Injected Quantity 1 µl |
| Column | Zorbax SIL 7 µ 100 x 4 mm |
|---|---|
| Eluent | A: CH2 Cl2 B: Methanol C: Water |
| Flow Rate | 1.5 ml/min |
| Injected Volume | 20 µl |
| Peaks | dR. Deoxyribose Rh. Rhamnose F. Fructose G. Glucose S. Sucrose R. Raffinose |
| Column | Asahipak NH2 250 x 4.6 mm |
|---|---|
| Eluent | A: Acetonitrile B: Water |
| Flow Rate | 1.0 ml/min |
| Injected Volume | 20 µl (2 ppm) |
| Peaks | F. Fructose G. Glucose L. Lactose S. Sucrose R. Raffinose St. Stachyose |
| Column | Lichrospher Diol 250 x 4 mm |
|---|---|
| Eluent | A: CH2 Cl2 B: Methanol |
| Flow Rate | 1.0 ml/min |
| Injected Volume | - |
| Peaks | DR. Deoxyribose Rh. Rhamnose F. Fructose Ma. Mannose G. Glucose S. Sucrose Me. Melibiose R. Raffinose |
| Column | Prevail Carbohydrate ES - 5 µm 150 x 4.6 mm |
|---|---|
| Eluent | Gradient MeCN / Water from 80:20 to 50:50 in 10 minutes |
| Flow Rate | 1.0 ml/min |
| Injected Volume | 5 µl – 50 ppm each |
| Column | Lichrospher CN 1250 x 4 mm |
|---|---|
| Eluent | CO2/MeOH |
| Flow Rate | 2.8 ml/min |
| Injected Volume | - |
| Peaks | a. Fructose b. Glucose c. Sucrose (saccharose) d. Raffinose |
| Column | Lichrospher Diol 250 x 4.6 mm |
|---|---|
| Eluent | CO2/Methanol (84.5/15.5) [w/w] |
| Flow Rate | 1.77 ml/min |
| Injected Volume | - |
| Peaks | dRi. Deoxyribose mE. Meso-erythritol Rh. Rhamnose Xl. Xylitol F. Fructose M. Mannose G. Glucose |
| Column | RSIL NO2 250 x 4.6 mm |
|---|---|
| Eluent | CO2/Methanol (87/13 [w/w] |
| Flow Rate | 3.8 ml/min |
| Injected Volume | - |
| Peaks | dRi. Deoxyribose Rh. Rhamnose F. Fructose G. Glucose Ml. Mannitol Sl. Sorbitol |
| Column | Imtakt UK-Amino 3 µm 2.0 x 150 mm, 60 °C |
|---|---|
| Eluent | A. Water / B. Acetonitrile |
| Gradient | 0 to 2 min: 10% A, 2 to 3 min: 10 to 20% A, 3 to 5 min: 20% A |
| Flow Rate | 0.8 ml/min |
| Injected Volume | 2 µl (500 ppm each) |
| Detector | SEDEX LT-ELSD 90LT, 30 °C, 3.5 bar |
| Peaks | Glycerol (0.78 min) Rhamnose (0.91 min) Arabinose (1.13 min) Fructose (1.31 min) Sorbitol (1.53 min) Glucose (1.85 min) Inositol (2.88 min) Sucrose (3.55 min) Maltose (4.30 min) |