AN1-5
A012Caffeine
Application Number : AN1
| Column | Ascentis Express HILIC 2.7 µm 2.1 x 150 mm, 30 °C |
|---|---|
| Eluent | A. Ammonium formate 200 mM pH 3 / B. Acetonitrile |
| Gradient | 0 to 1 min: 8% A, 1 to 8 min: 8 to 25% A, hold 4 min |
| Flow Rate | 0.5 ml/min |
| Injected Volume | 1 µl (500 to 1000 ppm each) |
| Detector | SEDEX LT-ELSD 90LT, 40 °C, 3.5 bar |
| Column | Zorbax TMS 7 µm 250 x 4.6 mm |
|---|---|
| Eluent | CO2/MeOH (89.3/10.7) |
| Flow Rate | 4.6 ml/min |
| Injected Volume | - |