AN1-1
AN1-3Glycerol
Application Number : AN1 (2 methods)
| Column | Imtakt UK-Amino 3 µm 2.0 x 150 mm, 60 °C |
|---|---|
| Eluent | A. Water / B. Acetonitrile |
| Gradient | 0 to 2 min: 10% A, 2 to 3 min: 10 to 20% A, 3 to 5 min: 20% A |
| Flow Rate | 0.8 ml/min |
| Injected Volume | 2 µl (500 ppm each) |
| Detector | SEDEX LT-ELSD 90LT, 30 °C, 3.5 bar |
| Peaks | Glycerol (0.78 min) Rhamnose (0.91 min) Arabinose (1.13 min) Fructose (1.31 min) Sorbitol (1.53 min) Glucose (1.85 min) Inositol (2.88 min) Sucrose (3.55 min) Maltose (4.30 min) |
| Column | Synchronis Amino 1.7 µm 2.1 x 100 mm, 30 °C |
|---|---|
| Eluent | A. Hexane / B. Acetone + 0.2% formic acid |
| Gradient | 0 to 1 min: 100% A, 1 to 6 min: 100 to 50% A, 6 to 8 min: 50 to 100% A |
| Flow Rate | 0.5 ml/min |
| Injected Volume | 2 µl (250 ppm each, glycerol 1000 ppm) |
| Detector | SEDEX LT-ELSD 90LT, 28 °C, 3.5 bar |
| Peaks | Tripalmitin (1.21 min) 1,3-Dipalmitin (4.90 min) 1,2-Dipalmitin (5.09 min) Palmitic acid (5.66 min) Monopalmitin (6.49 min) Glycerol (9.46 min) |