A097
A003
AN1-3Palmitic Acid
Application Number : AN1
| Column | Kromasil ODS, 5 µ (250 x 4.6 mm) |
|---|---|
| Eluent | A : 0.1% Aqueous TFA/MeOH (90/10) B : Methanol/Acetone (60/40) |
| Flow Rate | 1.0 ml/min |
| Injected Volume | 200 µg |
| Peaks | 1. Linolenic acid (18:3) 2. Linoleic acid (18:2) 3. Oleic acid (18:1) + palmitic acid (16:0) 4. Stearic acid (18:0) 5. δ-Tocopherol 6. γ,β-Tocopherols 7. α-Tocopherol 8. Stigmasterol |
| Column | Zorbax ODS 5-7 µm (alkyl bonded silica) 150 x 4.6 mm |
|---|---|
| Eluent | CO2/Methanol (97.6/2.4) |
| Flow Rate | 3.9 ml/min |
| Injected Volume | 700 ng of each compound in 4 µl THF |
| Peaks | 1. Lauric acid (C12) 2. Myristic acid (C14) 3. Palmitic acid (C16) 4. Stearic acid (C18) 6. Docosanoic acid (C22) 7. Tetracosanoic acid (C24) |
| Column | Synchronis Amino 1.7 µm 2.1 x 100 mm, 30 °C |
|---|---|
| Eluent | A. Hexane / B. Acetone + 0.2% formic acid |
| Gradient | 0 to 1 min: 100% A, 1 to 6 min: 100 to 50% A, 6 to 8 min: 50 to 100% A |
| Flow Rate | 0.5 ml/min |
| Injected Volume | 2 µl (250 ppm each, glycerol 1000 ppm) |
| Detector | SEDEX LT-ELSD 90LT, 28 °C, 3.5 bar |
| Peaks | Tripalmitin (1.21 min) 1,3-Dipalmitin (4.90 min) 1,2-Dipalmitin (5.09 min) Palmitic acid (5.66 min) Monopalmitin (6.49 min) Glycerol (9.46 min) |