A001
A050
AN1-1
AN1-2Sorbitol
Application Number : AN1 (2 methods)
| Column | Polypore Ca+2 and Zorbax ODS - 7 µm 250 x 4.6 mm |
|---|---|
| Eluent | Water |
| Flow Rate | 1.0 ml/min |
| Injected Volume | 20 µl |
| Column | RSIL NO2 250 x 4.6 mm |
|---|---|
| Eluent | CO2/Methanol (87/13 [w/w] |
| Flow Rate | 3.8 ml/min |
| Injected Volume | - |
| Peaks | dRi. Deoxyribose Rh. Rhamnose F. Fructose G. Glucose Ml. Mannitol Sl. Sorbitol |
| Column | Imtakt UK-Amino 3 µm 2.0 x 150 mm, 60 °C |
|---|---|
| Eluent | A. Water / B. Acetonitrile |
| Gradient | 0 to 2 min: 10% A, 2 to 3 min: 10 to 20% A, 3 to 5 min: 20% A |
| Flow Rate | 0.8 ml/min |
| Injected Volume | 2 µl (500 ppm each) |
| Detector | SEDEX LT-ELSD 90LT, 30 °C, 3.5 bar |
| Peaks | Glycerol (0.78 min) Rhamnose (0.91 min) Arabinose (1.13 min) Fructose (1.31 min) Sorbitol (1.53 min) Glucose (1.85 min) Inositol (2.88 min) Sucrose (3.55 min) Maltose (4.30 min) |
| Column | Hypersil GOLD HILIC 1.9 µm 2.0 x 150 mm, 40 °C |
|---|---|
| Eluent | A. Ammonium acetate 50 mM pH 5 / B. Acetonitrile |
| Gradient | 0 to 0.5 min: 5% A, 0.5 to 10 min: 5 to 30% A |
| Flow Rate | 0.5 ml/min |
| Injected Volume | 2 µl (500 ppm each) |
| Detector | SEDEX LT-ELSD 90LT, 35 °C, 3.5 bar |
| Peaks | Erythritol (2.45 min) Sucralose (3.61 min) Sorbitol (5.47 min) Neotame (6.04 min) Acesulfame K (6.79 min) Neohesperidin dihydrochalcone (7.35 min) Saccharin (7.92 min) Aspartame (8.45 min) Cyclamate (8.80 min) Rebaudioside A (9.68 min) |